An extended panel of LR MPPOL and HR IPPOL keratinocytes, and analysed citrate by targeted analysis applying gas chromatography and mass spectroscopy (Figure 9C). The outcomes reinforced the conclusions on the unbiased screen and showed that all eight HR IPPOL 16 of 22 lines had detectable citrate, while only D30 of the LR MPPOL had any, and all eight HR IPPOL had more than this (p = 0.006).Figure Levels of extracellular citrate are regularly elevated in HR IPPOL keratinocytes relative to LR MPPOL and Figure 9.9. Levels of extracellular citrate are consistently elevatedin HR IPPOL keratinocytes relative to LR MPPOL and regular keratinocytes. (A) The bar charts show citrate levels from the unbiased metabolic screen. The information the outcomes of normal keratinocytes. (A) The bar charts show citrate levels in the unbiased metabolic screen. The information are will be the results of three independent experiments normal deviation derived from one line of of wholesome regular keratinocytes, lines of three independent experiments +/-+/- normal deviation derived from one particular linehealthy standard keratinocytes, twotwo lines of LR MPPOL keratinocytes, D17 (p16INK4A -/-), and five lines of HR IPPOL keratinocytes. The data are concentrations not LR MPPOL keratinocytes, D17 (p16INK4A -/-), and 5 lines of HR IPPOL keratinocytes. The data are concentrations normalised for cell quantity or FP Inhibitor Accession protein, but are from cultures of equal surface area and presented as scaled intensity. White not normalised for cell quantity or protein, but are from cultures of equal surface area and presented as scaled intensity. bar = regular oral keratinocytes; stippled bars = LR PPOL keratinocytes; hatched bars = D17 (p16INK4A -/-) keratinocytes; White bar grey bars =oral IPPOL keratinocytes. Considerable by PPOL keratinocytes; hatched barsTest relative INK4A -/-) and dark = standard HR keratinocytes; stippled bars = LR one-way ANOVA (bar) or Welch’s = D17 (p16 to NHOK810 keratinocytes; and darkcell line bars) p 0.05;keratinocytes. Considerable by one-way ANOVA (bar) or Welch’s (A), but with (bars over individual grey bars = HR IPPOL p 0.01; and p 0.001. (B) The data are the same as for Test relative to NHOK810 (barssubtracted and normalised for cell 0.05; p 0.01; and presented (B) net scaled intensity/10ascells/mL. the background more than person cell line bars) p quantity. The data are p 0.001. because the information are the same 5 for (A), but Aurora B Inhibitor Compound together with the by 1 way ANOVA (straight bar). Welch’s Test was used to evaluate LR MPPOL (D6 and intensity/105 Substantial background subtracted and normalised for cell number. The data are presented as net scaled D30) with HR IPPOL lines D4, D9, by 1 way ANOVA (straight p Welch’s Test was utilized compare The bar charts show D30) cells/mL. Substantial D19, D20, and D35 linked bars) bar). 0.05; p 0.01; and pto 0.001. (C) LR MPPOL (D6 and citrate levels employing targeted metabolomics analysis. The data bars) p 0.05; involving a single (D30, and D17) and three (rest) with HR IPPOL lines D4, D9, D19, D20, and D35 linkedare the outcomes of p 0.01; and pE4,0.001. (C) The bar charts independent experiments +/- typical deviation derived The data are of benefits of between one particular (D30, (p16INK4A -/-), show citrate levels applying targeted metabolomics analysis.from four linesthe LR MPPOL keratinocytes, D17E4, and D17) and seven lines of HR IPPOL keratinocytes with the background subtracted and normalised for cell number. The data are and three (rest) independent5 experiments +/- sta.