Mice have been killed by cervical dislocation after isoflurane anesthesia. All experimental techniques acquired approval from the Service Veterinaire du Canton de Vaud

Grownup islets had been isolated as described . Neonates islets had been handpicked from collagenase-digested pancreas. Immunofluorescence microscopy examination of islets monolayers seeded on extracellular matrix-coated plates (Novamed, Jerusalem, Israel)…