Was nicely tolerated by mice and no abnormal behavior and unwanted effects have been observed throughout the experiment. Throughout the very first two weeks of the study, animals in all groups had a slight fat loss of 0.2.3 g, most likely due to the intragastric excitability (Figure 1). Soon after that, physique weight steadily increased throughout the study having a comparable rate across all groups so that no treatment-related difference was observed.Int. J. Mol. Sci. 2017, 18, 2110 Int. J. Mol. Sci. 2017, 18,three of3 ofFigure Body-weight transform of C57BL/6 mice treated with oral administration sea cucumber Figure 1. 1. Body-weight alter of C57BL/6mice treated with oral administration of of sea cucumber polypeptides (SCP). Mice received each day oral gavage of SCP at 0, 25, 50 or 75 mg/kg physique weight polypeptides (SCP). Mice received every day oral gavage of SCP at 0, 25, 50 or 75 mg/kg physique weight for for eight weeks. Values are suggests SD, n n = There was no considerable amongst control group group and eight weeks. Values are implies SD, = 10.ten. There wasano a substantial between control and SCP group by by repeated measures ANOVA (p 0.05). BW, body SCP group repeated measures ANOVA (p 0.05). BW, body weight.weight.2.2. Molecular Weight Distribution and Amino two.two. Molecular Weight Distribution and Amino Acid Composition Acid Composition As shown in Table 1, molecular weight distribution of SCP ranged from 100 to 2000 Da (94 ) As shown in Table 1, molecular weight distribution of SCP ranged from 100 to 2000 Da (94 ) (Table 1).Wnt3a Protein Gene ID Evaluation of amino acid composition of SCP indicated that glycine was probably the most abundant (Table 1). Analysis of amino acid composition of SCP indicated that glycine was essentially the most abundant amino acid present in SCP (18.54 g per one hundred g protein), followed by glutamic acid, alanine, arginine, amino acid present in SCP (18.54 g per 100 g protein), followed by glutamic acid, alanine, arginine, and aspartic acid, accounting for 11.23, 9.75, 7.55, and 6.92 g per 100 g protein, respectively (Table two). and aspartic acid, accounting for 11.23, 9.75, 7.55 and six.92 g per one hundred g protein, respectively (Table two).Table 1. Molecular weight distribution of Sea Cucumber Polypeptides (SCP). Table 1. Molecular weight distribution of Sea Cucumber Polypeptides (SCP). Molecular Weight Range 100 1.50 Molecular Weight Range one hundred 300 28.50 one hundred 1.50 300 600 30.00 one hundred 300 28.50 600 1000 20.00 300 600 30.00 1000 2000 15.50 600 1000 20.00 2000 3000 1000 2000 three.00 15.50 3000 1.50 2000 3000 3.3000 1.50 Table two. Amino acid composition of SCP. Table two. Amino acid composition of SCP. Amino Acid g/100 g Protein Gly 18.54 Amino Acid g/100 g 11.23 Protein Glu Ala 9.75 Gly 18.54 Arg 7.55 Glu 11.23 Ala 9.75 Asp 6.92 Arg 7.55 Pro 5.FGF-15 Protein Molecular Weight 90 Asp six.PMID:23991096 92 Thr 4.68 Pro 5.90 Ser 4.50 Thr four.68 Leu four.39 Ser 4.50 Val 3.83 Leu four.39 Lys 3.33 Val three.83 Ile 2.29 Lys three.33 Tyr two.10 Ile 2.29 Phe 1.80 Tyr 2.10 Met 1.77 Phe 1.80 His 1.57 Met 1.77 His 1.Int. J. Mol. Sci. 2017, 18,four of2.3. Impact of SCP on Cellular Composition of Spleen In comparison to the control, mice treated with 75 mg/kg SCP showed a substantial enhance in percent + and CD8 CD42.3. Impact of + cells, Cellular Compositionwith SCP 50 or 75 mg/kg also showed a larger % B cells SCP on and mice treated of Spleen + ) and NK cells (NK 1.1+ ) (Table three). There was no considerable distinction in percent regulatory (CD45R When compared with the manage, mice treated with 75 mg/kg SCP showed a substantial boost in T cells (CD4+ /CD25+ ) between mice treated with SCP.