Gi|183 980 221 gi|183 985 424 gi|183 980 745 gi|183 985 379 gi|183 983 668 gi|183 984 660 gi|183 985 108 gi|183 982 932 gi|183 985 378 gi|183 982 679 gi
Gi|183 980 221 gi|183 985 424 gi|183 980 745 gi|183 985 379 gi|183 983 668 gi|183 984 660 gi|183 985 108 gi|183 982 932 gi|183 985 378 gi|183 982 679 gi|183 985 410 gi|183 982 898 gi|183 984 791 gi|183 981 569 gi|183 983 350 gi|183 985 421 gi|183 980 929 gi|183 985 025 gi|183 980 785 gi|183 982 895 gi|183 982 952 gi|183 983name 10 kDa culture filtrate antigen EsxB hypothetical TGF beta 2/TGFB2 Protein MedChemExpress protein MMAR_5453 hypothetical protein MMAR_0722 immunogenic protein Mpt64 low molecular weight antigen Cfp2 hypothetical protein MMAR_4692 cold shock protein A CspA_1 hypothetical protein MMAR_2929 hypothetical protein MMAR_5548 hypothetical protein MMAR_2672 hypothetical protein MMAR_5439 PE family members protein cold shock protein a, CspA hypothetical protein MMAR_1553 transmembrane protein, MmpS5_2 6 kDa early secretory antigenic target EsxA (RANTES/CCL5 Protein Source EsaT-6) hypothetical protein MMAR_0908 lipoprotein DsbF PPE loved ones protein, PPE10 hypothetical protein MMAR_2891 hypothetical protein MMAR_2949 hypothetical protein MMAR_size (kDa) ten.6 5.7 15.0 22.7 12.two 12.3 7.two 8.3 four.two 8.9 3.7 4.5 7.two 14.5 9.1 ten.0 9.five 14.6 8.six ten.two 15.three 9.eight M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M. M.species marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum marinum M M M M M M M M M M M M M M M M M M M M M Mbottom-up information setb CE, CE, CE, CE, CE, LC LC LC LC LCLC CE, LCCE, CE, CE CE, LC CE,LC LC LC LCLC CE, LC CE, LC CE, LCRank is depending on E-value (E 9 10-4). bCE = present in bottom-up data set of secretome employing CZE; LC = present in bottom-up data set making use of LC.Figure 4. HCD fragmentation on the 10-kDa culture filtrate antigen EsxB. (A) Fragmentation spectra with the [M 7H] 7 charge state with HCD (normalized collision power was 28 ). (B) Sequence of this protein along with the fragmentation patterns observed with HCD.concentration range. To extend data to higher concentrations, we determined the conductivity of aqueous acetic acid and formic acid options by applying six kV across a 60 cm capillary filled with acetic acid and formic acid in water at concentrations ranging from 0.1 to one hundred and measuring current. Ohm’s law as well as the capillary geometry had been made use of to calculate conductivity, Figure 1 and Table S1 in the Supporting Data. Across all concentration ranges studied, acetic acid options have muchlower conductivity than formic acid. In addition, this data suggests that incredibly high concentrations of acetic acid (50 ) will have decrease conductivity than the 0.25 formic acid running buffer that is definitely typically utilised in CZE analysis of proteins. We also examined the present within a capillary filled with plugs of 70 acetic acid inside a capillary filled with 0.25 formic acid operating buffer. Plugs of acetic acid involving 0 and 27 cm in length had been injected into a 40 cm LPA coated capillary bydx.doi.org10.1021ac500092q | Anal. Chem. 2014, 86, 4873-Analytical Chemistry stress. The resistance from the capillary increased linearly with plug length, Figure 2. The resistance across the 40 cm long capillary was 1.four G when the capillary was filled with formic acid, plus the resistance elevated at a rate of 96 M per centimeter of injected acetic acid. These resistance values correspond to a conductivity of 1.5 mScm for 0.25 formic acid and 0.five mScm for 70 acetic acid; the conductivity of 70 acetic acid is roughly three times lower than the 0.25 formic acid separation buffer. These benefits sugge.