Product Name :
PF-4989216
Description:
PF-4989216 is a potent and selective PI3Kα inhibitor with a Ki of 0.6 nM.
CAS:
1276553-09-3
Molecular Weight:
380.40
Formula:
C18H13FN6OS
Chemical Name:
4-(4-cyano-2-fluorophenyl)-2-(morpholin-4-yl)-5-(1H-1, 2, 4-triazol-5-yl)thiophene-3-carbonitrile
Smiles :
N#CC1=CC(F)=C(C=C1)C1=C(SC(=C1C#N)N1CCOCC1)C1NN=CN=1
InChiKey:
MUENOTXSRZEFJV-UHFFFAOYSA-N
InChi :
InChI=1S/C18H13FN6OS/c19-14-7-11(8-20)1-2-12(14)15-13(9-21)18(25-3-5-26-6-4-25)27-16(15)17-22-10-23-24-17/h1-2,7,10H,3-6H2,(H,22,23,24)
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life:
≥360 days if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
PF-4989216 is a potent and selective PI3Kα inhibitor with a Ki of 0.6 nM.|Product information|CAS Number: 1276553-09-3|Molecular Weight: 380.40|Formula: C18H13FN6OS|Chemical Name: 4-(4-cyano-2-fluorophenyl)-2-(morpholin-4-yl)-5-(1H-1, 2, 4-triazol-5-yl)thiophene-3-carbonitrile|Smiles: N#CC1=CC(F)=C(C=C1)C1=C(SC(=C1C#N)N1CCOCC1)C1NN=CN=1|InChiKey: MUENOTXSRZEFJV-UHFFFAOYSA-N|InChi: InChI=1S/C18H13FN6OS/c19-14-7-11(8-20)1-2-12(14)15-13(9-21)18(25-3-5-26-6-4-25)27-16(15)17-22-10-23-24-17/h1-2,7,10H,3-6H2,(H,22,23,24)|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : ≥ 29 mg/mL (76.Menin-MLL inhibitor 21 medchemexpress 24 mM)|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥360 days if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|PF-4989216 (Compound 10) has excellent PI3Kα Ki (0.6 nM), good cellular potency (S473 IC50=79 nM), and good selectivity against mTOR (mTOR Ki=1440 nM). PF-4989216 has PI3Kα Ki less than 1 nM and mTOR Ki more than 1 μM. PF-4989216 also has excellent selectivity over 40 other kinases, and no major CYP inhibitions are observed. Less than 30% inhibition is observed in 1A2, 2C9, 2D6, and 3A4 CYP enzymes at 3 μM. The toxicity of PF-4989216 in several drug-sensitive and MDR cancer cell lines, including cells overexpressing ABCB1 or ABCG2, and in HEK293 cells transfected with human ABCB1 or ABCG2 is determined.Everolimus Biological Activity PF-4989216 inhibits human colon carcinoma S1 cell line and ABCG2-overexpressing subline S1-M1-80 with IC50s of 1.PMID:31536241 11±0.09 and 6.79±1.00 uM, respectively. PF-4989216 inhibits human breast carcinoma MCF-7 and ABCG2-overexpressing sublines MCF7-FLV1000 and MCF7-AdVp3000 IC50s of 2.30±0.68, 23.26±2.94 and 62.57±5.46 uM, respectively. PF-4989216 inhibits pcDNA-HEK293, ABCB1-transected MDR19-HEK293, ABCG2-tranfected R482-HEK293 cells with IC50s of 0.44±0.05, 0.38±0.06 and 5.05±0.89 uM, respectively.|In Vivo:|PF-4989216 (Compound 10) is dosed orally in our in vivo antitumor model, PI3K driven NCI-H1975 xenograft tumors. PF-4989216 demonstrates dose responsive tumor growth inhibitory activity from 25 to 200 mg/kg in QD oral dosing.|References:|Liu KK, et al. Highly Selective and Potent Thiophenes as PI3K Inhibitors with Oral Antitumor Activity. ACS Med Chem Lett. 2011 Sep 19;2(11):809-813.Products are for research use only. Not for human use.|